Love between People Essay

In the novel The Rescue by Nicholas Sparks, love is portrayed in many ways. By looking at the characters, Taylor McAden, Kyle Holton, Denise Holton and Mitch Kindle, it is clear that love is inevitable and although they try to ignore fate, it will be very difficult to overcome. This is shown through the lives of Kyle and Denise, Taylor and Denise, and Mitch and Taylor. The characters in the novel all reveal the theme of being rescued by love; as a result it proves that although there are people who try to ignore their fate, there are those who are willing to follow their fate, knowing that there will many difficult obstacles to overcome. Kyle and Denise are one of the many characters in the novel The Rescue to help portray the theme of love through their relationship with one other and their actions towards each other. Denise helps to reveal the theme throughout the novel with many different characters. Denise reveals the theme of love through her son Kyle, by the way she interacts with him, and this is also known, when Denise is thinking, â€Å"You’ve got to help me find my baby! He’s only four.† (Sparks 22). This quote states that Denise has a very close relationship with her son, Kyle and she cares very deeply for his well-being. This quote also states that since Denise is very concerned about finding her child, that she is a mother who has a special bond with her child and that she is a parent who is willing to do anything for her child. Kyle also helps to reveal the theme through his actions and words. This is known, when Kyle says, â€Å"I wuff you, Money† which is translated into â€Å"I love you, Mommy† (274). This quote states that Kyle indeed, loves his mother very much, because saying you love somebody, is the most precious thing you can say to somebody. The quote also states that Kyle is expressing his feelings and emotions to his mom, who he loves very much. Both Kyle and Denise help to portray the theme of love towards each other; this is also portrayed between the relationship between Denise and Taylor. Denise and Taylor are also one of the many characters who help to uncover the theme of love. They help to uncover the theme of love in their relationship towards each other sexually, and verbally. Denise bares the theme of love towards Taylor, by the way that they interact with each other, and their sexual relationship. This is known when Taylor tells Denise, â€Å"I care about you more than I ever cared about anyone† (269). This quotation states that Taylor really cares for Denise, and that he wants to let her know that he is there for her. Denise helps to reveal the theme, when she shows Taylor that she loves him and cares for him, when she gives him a kiss (278). That states that both Denise and Taylor have a deeper relationship, where the theme of love is expressed between the two of them more. Taylor and Denise both add on to help reveal that the theme of the novel is love. This is also portrayed through the relationship between Mitch and Taylor. Taylor and Mitch are another set of characters that help to reveal the theme of love through their relationship. The author helps to uncover the theme of love, through the relationship statues of the two males. This is known, when Mitch tells Taylor, â€Å"I’ll always forgive you Taylor†¦Ã¢â‚¬  (236) this states that Mitch loves Taylor so much that he is willing to forgive him no matter what he does. It also states that their relationship is very strong and that they are always there for one another. Taylor also helps to reveal the theme of love, when he says, â€Å"Mitch was like a brother to me† (246). This quote states that Taylor loves Mitch so much, that he considers Mitch as his brother; as part o his family. This proves that the author reveals the message through the characters, so that the readers can have a better understanding of what the theme is going to be, or about. In the novel The Rescue, the characters show that love is inevitable, and no matter what they try to do, it will always be there, and that there is no way to avoid it. Although love exists in the world, there are people in this world who will try to avoid their fate. It is inevitable, because there will always be love surrounding us, and no matter how hard we try to push it away and ignore it, it will always find its way back to us.

Adapting Community Interventions for Different Cultures and Communities Essay

The Mexican Americans had their own unique identity. They had their own style in each decade starting from the 1940s– including the Zoot Suit Riots, to the Chicano Movement, then to the 2000s –which is known as the LA punk looks. During the 1940’s, young Mexican Americans wanted to fight for their justice against the United States servicemen. They started a group and began to wear clothing that was out of the ordinary. They wore baggy dress pants and big drape jackets, including a hat. Because of their clothing, people called them the Zoot Suits but they referred themselves as Pachucos and Pachuca. According to the article â€Å"6 Decades of Mexican and Mexican- American Style Evolution† (Agrawal), there are 6 different decades of the Mexican- American style that include: the 1940s– the Pachuco look, the 1950s– the start of the Civil Rights Movement, where Rock music combined with fashion and included curled hair and polka-dot dresses for the women, the 1960s, the Chicano Movement and the Chicano Activists movement, the 1980s– which was shaped due to hip- hip and was known to be the Chola look, then the 1900s– which starred Selena, the Queen of Tejano music who became known due to her iconic style and became a well known music idol, and lastly, the 2000s. The 2000s allowed women to express their strength and solidarity through the LA punk style by keeping a simple makeup look. Although Mexican- Americans used their art through music, Chinese Americans believed in respecting their art through appropriation . Another significant time in US history was the Chinese Exclusion Act that included Chinese Immigrants. According to the article, â€Å"The Chinese Exclusion Acts: A Racist Chapter in U.S. Civil Rights History† (Chin), The Act that started in the year of 1882 prohibited: immigration on Chinese laborers, denying naturalization, and enforced Chinese laborers who are already legal in the United States and later want to re-enter and receive a certificate to return. The Chinese Immigrants worked as servants, fishermen, farmers, etc. They also worked on railroads and miners. Due to the Chinese Exclusion Act, it has turned the table around and changed history for Chinese immigrants and they were treated differently. They were treated differently because they were â€Å"different† from others by their looks and the way they dressed. Non- Chinese people would sometime be jealous of what Chinese Americans accomplished. Sticking to their cultures and backgrounds, the Chinese men wo uld usually have their hair long and in a ponytail, their clothing would consist of cultural robes, which is known as a Cheongsam. Cheongsam is worn as Chinese cultural appropriation for respect. Usually Chinese friends and family have to give the approval if you are allowed to wear a Cheongsam. Until today’s world, many people still stereotype not only Chinese people but Asian Americans in general due to what they wore back then because of their culture. Despite the appropriation, the Chinese Americans believed in, African Americans took a stand and did for what they believed in to achieve justice as well as the Mexican Americans. Going back into the 1900s, African Americans then segregated into urban housing. As they began to take over a community in New York City and begin with the Harlem Renaissance, which was located in northern Manhattan. During the Harlem Renaissance, several groups and organizations appealed to improvements economically and educationally. According to the article â€Å"The Harlem Renaissance a Cultural Rebirth† (Hall), after black soldiers engaged in World War I, they wished for a better America for themselves. This time period included African Americans making a great remark within their culture. Due to their remark, they also created a climatic change within their fashion statement. This fashion statement included: the Flappers, a masculine look for women, and day and night dresses. Flappers is another word for dancers because of the dresses they wore that made it easier for them to dance in. During this era, women decided to stand out and portray the masculine look. Women woul d want to wear men’s clothing and began to smoke, drink and drive, etc. Because of this movement, they created several movies until today’s world portraying â€Å"the Harlem Renaissance†. Some movies include Harlem Nights, Brother to Brother, Murder in Harlem, and the recent popular one that features similar actions of the Harlem Renaissance would be The Great Gatsby that features Leonardo DiCaprio. Although during the Harlem Renaissance was more of a fashion statement by making a remark, the Native Americans were quite different in terms of making their own fashion. During the time of the Wounded Knee Massacre, there were about 300 Native American deaths. There was a battle between the Native Americans and the United States soldiers in the Wounded Knee Creek in South Dakota. During this time Native Americans took belief in their Ghost Dance. The Ghost Dance was a religious dance to bring back their original world and to protect themselves. During this time Native Americans would have to hunt for animals to use their skin to make clothing and to stay warm. Hunting and gathering was a way for them to survive. In today’s society, American Indian culture has changed. As of now we usually see depictions of the American Indian culture in social media. Such as music videos, movies, or television shows that portray the American Indian culture. It has changed compared to back then. Their culture contains making art, clothing, jewelry, dance, and fashion. To the Native American culture, they typically don’t have a certain clothing they wear for appropriation. According to the article â€Å"Native by Design† (Montgomery), they believe that appropriation does not matter and what matters is representing where they came from and showing off their beliefs as much as possible. Urban Outfitters –the popular clothing company– brings off the Navajo vibe in every store. This includes the designs in the store, several pieces of their clothing, and the setting of the store. This represents that even though Native Americans had one culture back then, it doesn’t mean it has changed drastically. Throughout time, culture, beliefs, and traditions, we soon come to realize the true beauty behind it all. We realize the true beauty of different ethnic groups, especially of our own culture and beliefs. Yes, it will all change over a certain span, but it all depends on what we want to believe in and what we want to keep believing in. Till today’s society, the beliefs we used to believe in will become more and more modern as time goes by and it will evolve quickly before we know it. Stereotypes will change, beliefs will change, etc. It is best to understand the true beauty of one’s culture. Despite all the stereotypes and the modern technology, we should take in consideration of our own culture and admire what we have. Fashion will change each and every year but we just have to make the most of it and express ourselves through art. Like these four different Ethnic groups: Mexican Americans, Chinese Immigrants, African Americans, and Native Americans; they all believed i n different cultures and beliefs but each group used their own unique way to express what is theirs.

Health Insurance Analysis Report Essay Example | Topics and Well Written Essays - 1500 words

Health Insurance Analysis Report - Essay Example Therefore, ACA ensures quality health service is affordable to people regardless of their social class. The Act requires more individuals to get a health insurance coverage that will enable them access health facilities anytime without finance problems. As the ACA health reform seeks to achieve all this it will inject many changes in the health sector. This paper, therefore, addresses the impacts ACA has brought to the Health Sector. Patient to Physician Ratio After launching the new health reform on January 1, 2014, the entire healthcare system will shake from the huge number of individuals who will insure. Congressional Budget Office estimated that 14 million uninsured young people would join the group on January 1, 2014. By the year 2021, 16 million more will enter the brackets. When the newly insured people join the health system, the doctor workload will hike as the number of doctor visits will go up. Limited funding restricts people from visiting hospitals (America’s Hea lth Insurance Plans, 2007). Besides, the health reforms anticipated will increase claim cost and cause delays in return to work. In simple language there will be issues related to scheduling of doctors’ appointments, ensuring quality healthcare, and availability of medical resources (Gruber, 2002). According to American Journal of Medical Quality published a report and projected a shortage of nurses across America between 2009 and 2030, the USA will be hit by a serious shortage of more than 90,000 physicians by 2020 and by 2025, the shortage it will upsurge to more than 130,000 (Gruber, 2002). Lastly, the health reform recommends implementation of other measures of appointments with nurses and physical assistants, which the worker’s compensation system does not recognize. Eventually, this will cause compensation issues and add client consent requirements (America’s Health Insurance Plans, 2007). Besides, ACA aims at reducing those physician`s shortages by provid ing loan-based refund programs to principal doctors and offer incentives to medical schools to increase the number of medical students (Gruber, 2002). However, the healthcare system may not see the light of the day soon until students will be under this program graduate and begin their careers. Effect on Tax-Exempt Hospitals ACA has a goal of increasing transparency concerning the payback that tax-exempt hospitals get. The act requires those hospitals to conduct a Community Health Needs Assessment (CHNA) every three years to portray how they are carrying out their duties to meet needs of their patients (Gruber, 2002). Secondly the tax-exempt hospitals will be required to come up with a written financial assistance policy which would include criteria for eligibility to financial assistance, basis for coming up with figures charged to patients, and measures to be undertaken in case of nonpayment (America’s Health Insurance Plans, 2007). The ACA will keep those tax-exempt hospit als on toes to increase transparency for quality of care to the patients. To crown it all, the health reform recommends penalizing the tax-exempt hospital that does not meet those rules up to $50,000. Health Care Funding Through Affordable Care Act, the Health Centre Program will award new funding which consist of the following. First, Health Center Base Adjustments where the Health Resources and

The American Experiment Essay Example | Topics and Well Written Essays - 500 words

The American Experiment - Essay Example Jurists make their deliberations under the cloak of secrecy hence it is very difficult to make out what happens in their discussion chambers. Legal policymakers have for a long time relied on untested intuitions on juries to make important decisions about both the scope and conduct of jury trials. However, empirical research on the functioning of the jury is gradually replacing this initial reliance on intuition, speculation, and anecdotes. There is a lot of information on the cognitive processing at trial as well as dynamics of jury deliberations. In their research study, Zeisel and Kalven try to explain why and how members of the jury make their decisions (Murphy, Pritchett, Epstein & Knight, 2006). The authors refer to the whole jury system as an experiment but unfortunately with the conduct of serious human affairs. They describe how the Judiciary picks members of the Jury as one of the factors that make it an experiment. The authors state that the random choice of the Jurists and then the abrupt dismissal at the end of a trial makes it all the more experimental. They argue that most of the Jurists are not usually well versed with law terminologies and despite the Judge’s warnings; most of them often make their decision way early in the pre-trial phase. Zeisel and Kalven further state that the confidence in the jury system shows that the emotional and social aspects of the Jurists’ human nature were implicit in the formulation of this democratic ideal (Murphy, Pritchett, Epstein & Knight, 2006). According to their research, Jurists use two main phenomena when making their decisions, that is the punished-enough theory and decisions that have race as their basis. On the punished-enough theory, the authors argue that most Jurists make their decisions basing on their emotions rather than logic and the legal instructions that the Judge reads out to them at the beginning of a trial. They further use actual court records

Case study analyse - Strategic Management Essay

Case study analyse - Strategic Management - Essay Example Its mission is to add economic value to Chile’s products and services by promoting innovation and technology transfer activities, management techniques and human skills to natural resource-intensive sectors, aimed at taking better advantage of Chile’s natural resources and productive capacity. In 1997 Dr. Eduardo Bitran assumed the office as general director of Fundacià ³n Chile. He faced the difficult task of administering the institution, which was constituted as a private non-profit institution with 50% state ownership. One of the most innovative mechanisms that the foundation used, unique in Chile and probably in Latin America, was to create new businesses as a main means to diffuse and transfer technology. Dr. Bitran played a major role in Chile’s economic development. , Chile had managed to keep to its course and to maintain growth and stability amidst enormous turbulence, in a period of economic decline that had affected all of Latin America. Recognition of the importance of new knowledge as the base of future businesses was gaining importance and Venture capital funds became the focus. Local innovation clusters were formed around the wine and salmon industries, and specialized fruit production was beginning to reveal the benefits of biotechnology and sophi sticated production methods. Immediate challenge for Dr. Bitran was to identify the path and direction for Fundacià ³n Chile. His main challenge would be administer the institution to generate social benefits, as required by the mission entrusted to it as a quasi-public entity, while at the same time acting as a private business to obtain the necessary resources that would permit it to grow and develop. Fundacià ³n Chile’s promoted innovation and technology transfer emphasizing agribusiness, forestry and marine resources. It followed several modalities like demonstration businesses, technology transfer groups, training and diffusion, and providing services such as consulting to quality control.

Criminal justice Essay Example | Topics and Well Written Essays - 250 words - 1

Criminal justice - Essay Example The jury’s successful conclusion means that they have successfully found the defendant guilty or not guilty based on the testimonies and evidences presented to them. It is important to note that the jury’s successful conclusion is, in the same manner, the judge’s successful conclusion, not only the judge intervenes in the verdict made by the jury. The victim and the defendant’s successful conclusion means that the verdict favors them. This is similar to the prosecutor and defense attorney’s successful conclusion, wherein, it collides. Of course, the successful conclusion of the victim is when the court rules in their favor and puts the defendant behind bars, or worse. And the successful conclusion of the defendant is when the court rules in their favor and sets them free. A successful conclusion in the courtroom depends on the position, the side and the role of the participant. The judge and the jury are the one that dictates the successful conclusi on because they give the decision. But the victim and the defendant may have different views whether it is successful or not depending on how their testimonies and evidences are accepted by the court.

Innovation Business Essay Example | Topics and Well Written Essays - 750 words

Innovation Business - Essay Example Energy: Global energy requirement is growing continuously notwithstanding the exhaustible nature of conventional sources like coal, oil and natural gas, or even in the face of growth of renewable sources like wind, solar, geothermal, tidal etc. This trend will get accentuated as mankind breaks the shackles of earthly existence, to routinely make interplanetary travel to colonize the outer space. In this scenario, conventional meaning of energy or its quantities will become obsolete. What could be the characteristics of such energy? The new form of energy may be something from within rather than without. For example, a person walks by the mere intention of walking, aided by muscular power. Can the intention or the mental power, which is limitless, be made more important than the limited muscular power? Mental power is inherent to living things and I forecast that we will discover something similar in non-living things as well, and follow it up by innovations to energize it in a limitl ess manner. Inner-city transport: Personal and public transport as we see today is unsustainable as a factor of growth. Millions of cars on the ever-expanding streets/highways, or rail-transport systems moving masses of humanity, cry for alternative solutions for people living in metropolitan cities like Los Angeles, New York, London, Tokyo and Shanghai. The expensive fuel that is being spent and the exhausting nature of travel just to get to somewhere is the rationale for innovative ideas for inner-city transport. I forecast that a system of point-to-point moving platforms will substantially reduce roads/vehicles in a way that will combine the convenience of private vehicles and sustainability of public transport. Water: Water in oceans and other saline waters make up for the bulk of global water constituting 97.5% while only 1% of all the water on earth is fit for use by humans, according to the US Geological Survey; of this 1%, groundwater is 99% balance being surface water in la kes and rivers (n.d. web, USGS). Thus potable water is one of the very scarce but vital resources of earth, seriously impacting millions of people. As pointed out by USGS, the earth is a practically closed system, neither losing nor gaining any matter including water (ibid.). This natural phenomenon makes me forecast innovative recycling of the entire quantity of water that we use, at individual household level rather than the present practice of wastewater treatment and discharge by municipalities. Q.2 Threat of substitution GE’s innovative diamonds from coal are substitutes for DeBeers natural diamonds who enjoyed virtual monopoly in diamond trade till now. This situation changes the industry competition to oligopoly. Pure oligopoly markets are characterized by a few players producing identical products while in differentiated oligopoly, product features, styling, service etc. differ. GE’s synthetic diamonds are more economical and as the CEO of DeBeers, I can only m eet this price challenge by significantly reducing/destroying the profitability of the industry. Further more, there is no guarantee that GE’s success is not repeated by another firm with equal technical and financial clout, although there are not likely to be too many competitors of GE’s stature. Hence, price is not the weapon of combat. In the normal course, a customer perceives value of his purchase in terms of

Reflective Memo Essay Example | Topics and Well Written Essays - 500 words

Reflective Memo - Essay Example My first assignment on ‘Should Countries Control the Internet’ gave me an opportunity to discuss something, which I actually enjoy a lot. Discussions regarding the internet are usually my favorite. Additionally, in English 1302, writing skills such as free writing were commonly used. We also used peer reviews to help us generate ideas and put more emphasis on our writing. In as much as these sessions were challenging and complex, I can attest that there were signs of improvement when it came to generating ideas and putting them into writing. My grades kept improving during the course and that was reason enough I had improved in the course. A perfect example whereby I managed to utilize these new ideas during the writing process was while writing the project about ‘MTV’s 16 and pregnant’’ project. In doing the project, I categorized the entire project into different parts to make it easier to read and understand. Alternatively, I used electronic software in editing the entire project. In doing this, I copy pasted the word document in the software. After which, I revised the draft while still in the software. By the time I completed this process, I had done extra work on the project before posting it on the writing studio. I chose the ‘MTV’s 16 and pregnant’’ project because of its perfect illustration when it comes to the outcome. Over the semester, I have been trying my best to improve in the English 1302 course and the ‘MTV’s 16 and pregnant’ ’project, remains one of the best pieces of work I have done during the course. The entire draft is self-explanatory and does not require explanations. However, the first paragraph is a bit detailed since it is the face of the paper. The first draft generally explains all about Lowrey Annie through various themes. The main theme in the first paragraph is about some of the challenges, which teen mothers have to go through. The paragraph does not only focus on Lowrey Annie but

Evacuation Procuedures & Strategies in Buildings Dissertation

Evacuation Procuedures & Strategies in Buildings - Dissertation Example As the sky scrapers rose to majestic heights, so did the threats to their occupants. Evacuation procedures have constantly been under revision ever since the first evacuation plan was devised. Threats in terms of global hazards, accidents and recently terrorism have taken their toll and thousands of people have perished to date due in events such as fires in high rises, buildings collapsing due to earthquakes and terrorism incidents. The study is aimed to bring a deep insight from current evacuation procedure in high rises in light of new global and internal threats emerged in the last decade. Moreover, the study presents a brief overview with challenging issues in evacuation procedures in Burj Al Khalifa and Taipe Tower II. Table of Contents Proposal Methodology 1. INTRODUCTION Need of high rise building 2. LITERATURE REVIEW A. Events that may Lead to Evacuation I. External Threats a) Tsunamis b) Earthquakes c) Floods d) Cyclones and Hurricanes e) Heavy rains in Jeddah II. Internal Threats a) Fire b) Power Failure III. Terrorism and Accidents a) Air plane crash b) Terrorist attacks (bomb blast) B. Means of Alerting the Occupants in case of an Emergency C. General Problems in any Evacuation System I. Training of Floor Wardens II. Distribution of Handouts III. Seminars and Awareness Sessions IV. Evacuation Drills D. Severity of threat to High Rises 3. CHALLENGES IN EVACUATION A. Evacuation plan depends on the nature of the disaster I. Total Evacuation II. Select Evacuation III. Protect in Place B. Real-time evaluation of the problems during evacuation I. Problem sensing II. Identification of regions at high risks III. Calculating/Computing the most optimize evacuation plan in real time 4. AREAS OF CONCERN WHEN DESIGNING A HIGH RISE BUILDING A. Time Constraint B. Complex building architectures 5. AVAILABLE SOLUTIONS A. EVACUATION PROCEDURES AT BRIDGE AT KHALIFA I. Structural details II. Safety Measures Incorporated in the Design and Construction B. EVACUATION PRO CEDURES AT TAIPEI 101 I. Structural details II. Safety Measures Incorporated in the Design and Construction 6. CONCLUSION/DISCUSSION 7. REFERENCES PROPOSAL Tall buildings are being constructed all over the world. Almost all the urban skylines are pierced by sky scrapers reaching out into the heights as a testimony to human development in the fields of civil and mechanical engineering as well as the technological accomplishments that make the implementation of those developments possible. Since the appearance of the first high rise building on the globe in 1890 i.e. The World Building in New York City this structure was 309ft high, the tall buildings have grown taller and now expected to exceed the 1km height milestone. The construction of high rise buildings brought a new trend in the residential as well as office buildings. Now companies and service providers seek offices and work places in distinguished structures that can play a positive role in their marketing and public relatio ns campaigns. A certain part of the residential community, though not the majority, also look forward to having residences in distinguished buildings, preferably tall buildings. A sky scraper of moderate height i.e. 300m to 400m can house around 2500 to 2800 people in the event of an emergency it will never be easy to evacuate all of the occupants safely in time, and to do so will require great planning supported by safety features incorporated into the building’s design during construction. There are many advanced safety

Are juvenile more likely to be dysfunctial from single parent homes Essay

Are juvenile more likely to be dysfunctial from single parent homes - Essay Example Reynolds has referred to the comment of Yoshikawa (2004) that while the average expense for pre school intervention for a child is about 5000 USD, the average yearly expense for juvenile detention or incarceration is more than 30,000 USD and the results are not guaranteed in the latter case. Inner city region of Chicago was chosen as the program was implemented in the poorest neighbourhoods that have been associated with high unemployment, low social control and low quality schools that are predictors for delinquency and anti social behaviour. Reynolds (2008) points out that the tests conducted where based on certain indicators of delinquency such as school attendance, promiscuous behaviour, theft, aggressive behaviour and fighting and so on. Parents were encouraged and asked to attend counselling sessions for children. The author reports that delinquency incidents for children where both parents attended the sessions and homes where both parents were present showed a reduction of 30%. On the other hand, children who came from homes with a single parent, typically the mother who worked the whole day showed a remarked reported increase in the incidents of delinquency by as much as 47%. There were other factors such as education of the parents, income levels, if the single parent was an alcoholic or drug addict and so on. While the research was very professionally conducted, the author has hypothesised that Juvenile Delinquency is only in poor and black neighbourhoods. Studies should also be performed among middle class white youths to form a relational study that children from single parent homes have a higher tendency to commit juvenile delinquency incidents. Demuth (2004) has researched the occurrence between juvenile delinquency and single mother family versus two-parent family and single father family. This study was designed to identify the means by which single parent increases delinquency and if effect is mainly a function of parental

World Wide Web and Internet Essay Example for Free

World Wide Web and Internet Essay The Internet is certainly the place for businesses to expand, reach new markets and provide new services but there must be a balanced approach to the Internet. The Internet must be regarded as a strategic resource important to the whole business. The technical detail of the Internet can be complex. Management responsibility however is more productively focused on managing the implementation process to achieve strategic goals and assure smooth, secure operations. The IS Manager must first define why the company wants to be connected to the Internet then move to establish the appropriate connection, choosing the most cost effective option between capacity and speed. For medium businesses it is recommended that high speed modem links to a provider with dedicated telephone lines would be most appropriate. Issues such as how to advertise and promote the company on the Web must then be tackled, taking into account the human and social issues on the net. Users of the Web can include both employees and potential customers. By learning what and how the Internet can help the employees in their day to day work, non-business use of the Internet can be reduced, and by offering on-line services and supporting the customer via the Internet, a whole new market can be created for the business. A medium business would consider having a Web site with the Internet provider but perhaps creating and updating the web pages themselves. Finally security against intrusion has to be maintained. Technology is a vital tool to IS Managers to cope with this. Also by having the Web site at the provider and linking to the Internet by regular telephone lines it makes the companys data safe from hackers. In conclusion, with careful planning and proper management, putting a business on the Internet can make it more effective and productive. However understanding and knowing how the rapid changes and various complex issues that can be encountered in getting the business onto the Net is imperative to its success. This responsibility lies largely on the IS Manager who must know what options are available to him or her before being able to cope with it. Surfing the internet can be an interest for people. But people need to use it properly. If not, it will become internet addiction. Internet addiction harmfulness is bigger than â€Å"TV addiction†. In deed internet addiction can be called â€Å"a mental illness†.Playing online games reasonably can make people happy on free time.

20 Years Ago India Essay Example for Free

20 Years Ago India Essay Twenty years ago this weekend, three top Indian officials burned the midnight oil tearing up old import controls and preparing a package of economic reforms that would slowly lead to the booming India that is widely admired today, with growth of 8-9%, 300-350m people enjoying the benefits of a consumer economy, and businessmen operating internationally. But India seems to be in no mood to celebrate that momentous event, just as it wasn’t at India’s 50th anniversary of independence in 1997 when the feeling was downbeat. People then were unsure of what to celebrate, since so little had been achieved in terms of economic development, care for the poor, and industrial efficiency since the British left in 1947. Ten years later, that had changed because of the economic boom of the intervening years. But the 1997 mood is now back again. People are aware that, despite all the economic and business successes, 800m people are still desperately poor and under-nourished, with poor access to clean water and health and education services. Public infrastructure and services are crumbling, national security and defence preparedness is woefully inadequate, and governance is sliding into a greedy, corrupt and inefficient abyss with no bottom in sight. Popular contrasts of India’s elephant and China’s tiger economies are being trotted out in various articles and studies, as they have been for 20 years. But the contrast is simplistic because India has its tiger industries such as information technology (IT), autos, pharma, and mobile telecoms that have been spurred by entrepreneurial drive and technological change. There are also rapidly industrializing states – notably Gujarat and Tamil Nadu (despite its political corruption). These are taking the place of India’s earlier internationally lauded cities, Bangalore and Hyderabad, the capitals of Karnataka and Andhra Pradesh that have been swamped by the greed and corruption of politicians and businessmen in areas such as land acquisition, mining and real estate. (The Karnataka chief minister is this week accused of facilitating multi-million dollar illegal mining). India’s blundering elephant is the government establishment that has refused over the past 20 years to change the way that the country is run. The 1991 whittling-down of the government’s role has not been followed through. The government still controls the mostly unreformed banking and defence sectors as well as the vast array of public sector industries and, in various ways, land useage and licensing, especially in the corrupt telecom sector. Such government controls skew development. When the current United Progressive Alliance (UPA) came to power in 2004, led by Sonia Gandhi and Manmohan Singh, reforms were initially held back by Communist-led Left Front that supported the government. Since the 2009 general election, reforms have been blocked by the disproportionate power of other coalition partners that have 20 or fewer MPs out of the coalition’s total of 262. The main problem however is that Sonia Gandhi, who heads the Congress Party, is not a firm enough believer in reforms to push Singh and his government into a tougher line, and Singh is too cautious. Consequently, a raft of reforms have been delayed including divestments of stakes in public sector businesses, increasing FDI in various sector such as defence, insurance and retail, and – most important of all – curbing subsidies. Montek Ahluwalia, whose Planning Commission is currently finalising a new five-year plan, argues that the future focus should be on three more urgent areas that would otherwise block economic progress – the use of energy and water, and urbanisation. These areas need changes of action by the central government, and even more by state governments, that has eluded India for the past 20 years. It is hard to see how India can tackle these issues, given that failure since 1991. People who are well off will of course do better, and the 300-350m people now enjoying varying levels of consumerism will increase in number and satisfaction. Companies will become more profitable and will become more internationally active. But social tensions will increase, with growing battles over the use of land and other scarce resources. Major reforms will be needed to reverse the trend of bad governance and corruption. It is an irony that, though the past 20 years began and now end with Manmohan Singh, he was neither in charge at the beginning, nor is he at the end. That is not a criticism, but in the early 1990s he could only do what he did courtesy of Narasimha Rao, and now he cannot do what he doesn’t do courtesy of Sonia Gandhi and the UPA’s coalition partners. Something surely needs to change.

Electron Transport Chain in Respiratory Complex I

Electron Transport Chain in Respiratory Complex I Introduction Every organism depends on energy to survive, in order to maintain an organized state, homeostasis, through metabolism and other biochemical reactions. Energy is generated in a number of different ways depending on the organism. Mammals create energy through the breakdown of organic molecules, such as carbohydrates, proteins and lipids, that yields other compounds that drives cellular processes. One such compound is ATP (Adenosine triphosphate) an essential energy-carrying molecule that is synthesised by respiration through a series of enzyme protein complexes found in the mitochondria. Complex I (NADH:ubiquinone oxidoreductase) is one of those essential protein complex embedded in mammalian mitochondria. NADH produced by the Krebs tricarboxylic acid cycle and ÃŽÂ ²-oxidation of fatty acids, is oxidised to initiate the mechanistic pathway of Complex I, ultimately reducing ubiquinone and establish proton-motive force across the inner membrane of the mitochondria. It is this proton g radient that will support the generation of ATP from ATP synthase and other core processes. Significant research has been conducted on Complex I, particularly from Bovine heart mitochondria, however to date many aspects of this enzyme is still poorly understood due to its complex structural arrangement and pathways undertaken. To decipher its mechanism, will eventually lead to a greater understanding in the role of Complex I in many diseases and dysfunctions. Mitochondria Mitochondria are small sub-cellular organelles involved in a series of processes primarily with its role in the respiratory system. Occupying almost 10% to 30% of cell volumes of sizes ranging between 0.75 and 3ÃŽÂ ¼m, the unique shape of a mitochondrion allows the process to take place, with its key structural feature being a double membrane.1 These two membranes are separated by the intermembrane space and overall enclose the central matrix. Whereas the outer membrane is inundated by porins to facilitate the movement of solutes of about 12 kDa or less; the inner membrane is impermeable to solutes but presents the ideal environment for the establishment of an electrochemical proton gradient, by the presence of numerous protein complexes. Additional compartments of the organelle include the cristae and the mitochondrial matrix, which comprises a plethora of enzymes involved in ATP metabolism. Additionally, a range of studies have also indicated the ability of mitochondria to form dynamic networks of interconnected tubules that regulates the cell structure to adapt to its specific function when required. As a result, during disruption of such networks, cellular dysfunction can occur, leading to a number of neural related syndromes such as Parkinsons and Alzheimers.2,3 Aside from the primary role of energy metabolism, the mitochondria also power other core cellular functions such as apoptosis, calcium handling and the formation of iron sulphur clusters. The following sections discuss the main enzymes involved in the electron transport chain that lead to the generation of ATP, particularly respiratory complex I, which will be the main focus of this thesis. Respiratory Complexes Complex II Also known as succinate: ubiquinone oxidoreductase, complex II is a 120 kDa enzyme consisting of four nuclear-encoded subunits which are arranged in two domains.4 It is this distinctive arrangement which allows this enzyme to oxidise succinate to fumarate which is coupled to the production of ubiquinol through the reduction of ubiquinone in the mitochondrial inner membrane. While it is involved with cofactors, this enzyme complex does not directly contribute to the proton motive force in order to establish a chemical gradient.4,5 Succinate+ Q à ¢Ã¢â‚¬  Ã¢â‚¬â„¢ Fumarate + QH2 Equation 1: Two of the enzymes subunits SdhA and SdhB form a hydrophilic, succinate dehydrogenase subcomplex and forms the succinate/fumarate binding site whereas SdhB contains three iron-sulphur clusters which are embedded to the mitochondrial membrane by the remaining SdhC and SdhD subunits.4 These latter subunits contain a heam group and ubiqionone binding sites. When a flavin dinucleotide, which is ligated to SdhA, it oxidises succinate, the electrons produced in this process are passed down through the iron-sulphur clusters. The electrons subsequently allow the reduction of ubiquinone to ubiquinol.6,7 Complex III Complex III or ubiquinol:cytochrome c oxidoreductase is an 240 kDa enzyme which is made up of 11 subunits. Its structure comprises of two ubiquinone binding sites; Qo, present towards the mitochondrial membrane, catalyses the oxidation of ubiquinol to ubiquinone and Qi, present towards the matrix, catalyses the reduction of ubiquinone to ubiquinol.8,9 Complexes I and II produces ubiquinol from the reduction of ubiquinone, which binds to the Qo site on complex III. During this process, an electron is passed along the iron-sulfur cluster reducing it and moving it towards cytochrome c1 and cytochrome c resulting in a conformational change. The change causes a second electron to be transferred through another pathway formed of cytochromes bL and bH towards to Qi binding site, in where it allows the formation of a semiquinone anion through the reduction of an already bound ubiquinone. Parallel to this, a second quinol is oxidised at Qo allowing the electron to be transferred through the first pathway of Rieske iron-sulphur cluster and cytochrome c1 and the second electron follows the second pathway mentioned above to Qi, reducing the semiquinone anion to ubiquinol.10 The oxidation at Qo releases four protons into the inter-membrane space of the mitochondria and the reduction at Qi results in the uptake of two protons from the matrix wh ich are transferred into the inter-membrane space during ubiquinol oxidation. This complete cycle allows the reduction of two cytochrome c molecules.9 QH2 + 2 cyt c3+ + 2H+in à ¢Ã¢â‚¬  Ã¢â‚¬â„¢ Q + 2 cyt c2+ + 4H+out Equation 2: Oxidation and reduction cycles in Complex III results in the movement of four protons into the inter-membrane space maintains the proton motive force used by ATP synthase to synthesise ATP.8 Complex IV Complex IV, also known as cytochrome c oxidase, is an enzyme, which comprises of 13 subunits, of which three are encoded by the mitochondrial genome. The enzyme catalyses the oxidation of cytochrome c which leads to the reduction of oxygen to water allowing the translocation of four protons across the mitochondrial inner membrane.11,12 The oxidation of cytochrome c produces electrons that are transferred to an active site where molecular oxygen is reduced. This reduction producing water releases free energy required for the pumping of four protons from the matrix of the mitochondria into its inner-membrane space. This movement of protons is facilitated through two known proton channels: the K-channel passes two protons for the reduction of oxygen and the D-channel allows the movement of newly translocated protons.13 O2 + 4 cyt c2+ + 8H+in à ¢Ã¢â‚¬  Ã¢â‚¬â„¢ 2 H2O + 4 cyt c3+ + 4H+out Equation 3: The translocated protons and the reduction of oxygen to water allows ATP synthase to generate ATP as this contributes to the proton motive force similar to Complex III. Complex V Primarily known as ATP Synthase, this enzyme complex operates by utilising the proton chemical gradient established in the intermembrane space by the preceding complexes, to drive the synthesis of ATP from ADP and inorganic Phosphate. With an average size of 580 kDa, the enzyme is composed of 16 subunits organised in two hydrophobic and hydrophilic domains; the hydrophobic domain forms a proton conductive pore through the inner membrane while the hydrophilic domain, containing three copies of ÃŽÂ ± and ÃŽÂ ² subunits, spreads into the matrix. The two domains are linked by an asymmetric central stalk and a peripheral stalk, which acts as a stator to prevent the F1 domain rotating freely during catalysis. The interfaces between the two subunits forms the binding sites for ADP and inorganic Phosphate. 14,15 ADP + P+ nH+in à ¢Ã¢â‚¬  Ã¢â‚¬â„¢ ATP + nH+out Equation 4 Complex I Complex I, is the first and largest enzyme involved the electron transfer chain of the mitochondrion. Alternatively known as NADH:ubiquinone oxidoreductase, its primary role is to oxidise NADH and ultimately reduce ubiquinone.16 NADH + H+ + Q + 4H+in à ¢Ã¢â‚¬  Ã¢â‚¬â„¢ NAD+ + QH2 + 4H+out Equation 5: Just like the other protein complexes, the potential energy released from the redox reaction within the complex, translocates four protons across the inner membrane for every molecule of oxidized NADH and removes two additional protons from the matrix for the reduction of quinone. The processes contribute to the overall electrochemical gradient which is to be used by ATP synthase to synthesise ATP.17 Structure To date, complex I has been found in a variety of species, including many prokaryotes. The complex I from bovine heart mitochondria is primarily used in studies due to its close sequential identity with the human complex I enzyme. The mammalian complex I is one of the most complex and largest enzymes known, with a combined mass of 980 kDA and composed of at least 45 different polypeptide subunits; with 14 strictly conserved core subunits that are necessary for function and also common across the among all known complex I.16 The significance of the additional subunits in complex I among different species still remain a mystery. It is known some be involved in protection against reactive oxygen species generation and some are required needed for proper assembly and stability of the enzyme.16,18 As observed by single-particle electron microscopy (EM) for both bacterial and mitochondrial enzymes, the determined structure of the enzyme closely resembles to an L shape, with seven hydrophobic core subunits that constitutes the membrane tail domain and seven hydrophilic core subunits that constitutes peripheral (hydrophilic) arm domain protruding into the mitochondrial matrix; which is known as the catalytic domain as it includes all redox centres and binding site while the membrane domain consists mostly of hydrophobic subunits. 16 While the full structure of the eukaryotic complex is not still well characterised, in 2006, Sazanov group successfully reported structure of the hydrophilic domain of complex I from Thermus thermophiles bacteria.20 The Peripheral Arm of complex I The peripheral arm of the complex is composed of seven individual subunits, that together, houses the NADH-oxidizing dehydrogenase module, which provides electron input into a noncovalently-bound flavin mononucleotide (FMN) molecule. The molecule sequentially transfers the electron to a chain of nine iron-sulphur (Fe-S) clusters, eight of which are found in the bovine enzyme. Additionally, the hydrophilic arm also comprises of a Q-module, which conducts electrons to the quinone-binding site for quinol production. 16,20 All of these Within the respiratory chain complexes, there are three different types of Fe-S clusters, two of which, are found in complex I; Two binuclear [2Fe-2S] and six tetranuclear [4Fe-4S] clusters. As the name suggests, the binuclear clusters are composed of two iron atoms that function as bridged by two acid-labile sulphur atoms. Each iron atom is also coordinated by an additional two sulphur atoms found on the surrounding cysteine residues from the protein complex. In the tetranuclear Fe-S clusters, four iron atoms and four sulphur atoms are arranged in a cube with each iron atom also ligated to sulphur cysteine-residue on the surrounding protein, similar to binuclear Fe-S.22 Due to their conformational arrangements and redox capabilities provided by the iron atom, these clusters act as electron transfer agents or also known as ferrodoxins. The detection of these clusters can be achieved by EPR (electron paramagnetic resonance) which is successfully achieved in many studies. However, out of the two binuclear and six tetranuclear iron-sulfur clusters found in complex I, only two binuclear and four tetranuclear clusters are EPR active.22 Figure 1.: structures of the iron-sulphur clusters found in complex I. As previously mentioned, seven of the eight clusters, form a 95 Ã…-long extensive chain directly from the flavin site to the quinone binding site on the interface of the membrane domain. Even though the distances between these chains may seem far apart, as much as 14 Ã…, distances are close enough to allow electron transfer to occur.23,24 However, the presence of the eight cluster is still not well understood. Cluster 2Fe[24] found on the opposite side of the Flavin site, is believed not to be involved in electron transfer pathway. While it was just a theory with no evidence, it has been proposed that this additional cluster functions as an electron store that accepts an electron from the flavosemiquinone species preventing the generation of reactive oxygen species during enzyme turnover.24 Membrane Domain of complex I The membrane domain comprises the proton-translocating module which catalyses proton transport. With the exception of subunit ND1 and the quinone binding site, found on the interface of the peripheral arm, the membrane domain functions totally independently from the two arms of complex I. Within the membrane domain, there are four structural subunits that have been identified to be possibly involved with proton translocation; these include subunits ND2, ND4 and ND5. There is also an additional transporter which believed to be either ND1, ND6 or ND4L. Each believed to be transporting one proton per catalytic cycle. Each individual subunits are composed of charged residues and helices that creates half-channels that allow the passage of proton to occur. The membrane structure is also held together by a long ÃŽÂ ±-helix chain that spans across its entire length. Its feature is to maintain and support the integrity of the membrane domain.26 Overall Mechanism of complex I The mammalian complex I includes 45 known proteins, out of which 14 core subunits comprises of both hydrophilic and hydrophobic domains as explained above.16 The mechanism through the electron transfer chain starts with a Flavin mononucleotide (FMN) molecule which is non-covalently bound to the 51kDa subunit through hydrogen bonds at the top of the hydrophilic domain. FMN molecule oxidises NADH leading to the reduction of iron-sulphur clusters (Fe-S) which transfers electrons from Flavin to the quinone-binding site {51}. This electron transfer distorts the conformation of the protein through changes in its redox state leading to alterations in pKa values of its side chains; these alterations allows four hydrogen ions being pumped out of the mitochondrial matrix.24 It is believed NADH gets oxidised to NAD+ through a hydride transfer avoiding the formation of the unstable NAD. Radical.24 This oxidation process occurs when the nicotinamide ring of the NADH lies above the flavin isoalloxazine system, allowing the electron donor hydride (C4 of the 27 nicotinamide ring) and acceptor (N5 of the flavin) to come within 3.5 Ã… of each other and transfer electrons.28 As explained above, NADH oxidation leads to transfer of electrons through seven iron-sulphur clusters chain between Flavin and quinone reduction binding site in the membrane.20 It is the final Fe-S cluster that donates the electrons to the bound ubiquinone substrate which is believed to be accessed through an entry point in the membrane to the binding site.21 These iron-sulphur clusters are best detected using a technique called electron paramagnetic resonance (EPR). Previous studies have observed five reduced Fe-S clusters through EPR from Bovine compliex I reduced by NADH, and their spectra are represented N1b, N2, N3, N4 and N5.25 This technique will be further explained throughout this thesis. A much recent study by Roessler et al. (2010) used EPR to understand the tunnelling electron transfer pathway through these clusters. Previous studies have already established EPR signals N1b, N2 and N3 are detected from 2Fe cluster in the 75 kDa subunit (position 2), and from 4Fe clusters in the PSST (position7) and 51 kDa subunits (position 1) respectively along the clusters chain due to interactions with ubisemiquinones and flavosemiquinone. As the other EPR signals have yet failed to be assigned to a particular cluster, Roessler et al. (2010) went on to use double electron-electron resonance (DEER) spectroscopy to detect N4 and N5. Their results demonstrate that N4 is assigned to the first 4Fe cluster in the TYKY subunit (position 5), and N5 to the all-cysteine ligated 4Fe cluster in the 75 kDa subunit (position 3).25 The study propose an alternating energy potential profile for electron transfer along the chain between the actives sites, in B.taurus, which enhances the rate of a single electron travelling through the empty chain subsequently leading to more efficient energy conversion in complex I.25 Followed by the iron-sulfur cluster is the site of quinone reduction. A study performed by Sazanov and Hinchliffe has identified a supposed binding site for the quinone head group from T. thermophilus complex I hydrophilic domain between the 49 kDa and PSST subunits.20 This alleged site is close to the cluster where the ubiquinone substrate accepts electrons from the chain and it has also been acknowledged the 49 kDa and PSST subunits play an important role in quinone binding and catalysis.29 Nevertheless, it is believed that additional hydrophobic subunits may also be involved in quinone binding and these are still being investigated. Even though the mechanism of NADH oxidation and ubiquinone reduction is relatively well understood, how this oxidoreduction leads to quinone reduction and subsequent protons pumping across the mitochondrial membrane from complex I still remain a mystery. A number of theories for complex I mechanism have been proposed based on the proton-pumping systems of the other mitochondrial respiratory complexes. These theories have been outlined below: A direct coupling mechanism as demonstrated by complex IV through cytochrome c oxidase where the proton transfer is determined by a gating reaction occurring at the same time as the electron transfer reaction that started it.30 An indirect coupling mechanism as seen in complex V (ATP synthase) explained previously. A study performed by Efremov et al., suggests that within complex I, one proton is translocated by a directly coupled mechanism at the Fe-S clusters and the rest are moved when quinone reduction drives conformational changes to the four-helix bundle of Nqo4 and of Nqo6 in complex I, subsequently affecting the C-terminal helix of Nqo12. The C-terminal has been identified by the authors running parallel to the membrane. The effect on this helix consequently leads to the other three helices to tilt which results in proton translocation.31 A Q-cycle-like mechanism as represented by complex III where quinol is used as a carrier to transport protons across the mitochondrial membrane. A study completed by Dutton and co-workers suggested the complete reverse of this mechanism for complex I featuring the presence of two ubiquinone binding sites; one facing the inter-membrane space, Qo, and the other facing the mitochondrial matrix, Qi. The quinone substrate would bind at Qi, and be reduced by one electron from a quinol already bound at Qo and another electron from the Fe-S cluster; subsequently leading to two protons being taken up from the matrix while the formed semiquinone specie is still bound at Qo. Following the uptake of the protons, semiquinone is oxidised to ubiquinone.32 Nevertheless, further studies conducted have found no evidence of ubiquinol oxidation signifying complex I do not work through this mechanism.30,33 While the first isolation of complex I from bovine heart mitochondria by Joe Hatefi et al occurred 40 years ago, information on its overall mechanism of action is still very limited particularly the mechanism of redox-proton coupling occurring in the membrane domain. To further understand this, new studies are being conducted to trap radical intermediates formed at the interface of the peripheral and membrane arm to establish the pathway that initiates proton translocation. Semiquinone radicals Semiquinones are catalytic intermediates formed within complex I during the reduction of quinones at the quinone binding site and can exist in neutral or anionic form. Due to the presence of the unpaired electron, semiquinone intermediates can be studied using EPR spectroscopy. There are numerous pathways in which the formation of semiquinones can occur from quinone. The scheme below, proposed by Roessler and Hirst, illustrates the three main possible routes taken to obtain quinol. Pathways A and B involves with the generation of a neutral semiquinone radical specie based on the transferring of a proton and electron. On the other hand, pathway C which follows through pathway B involve with the generation of an anionic radical specie generated from an electron transfer. All pathways lead to formation of quinol by series of electron transfer and protons. The pathway shown in grey which occurs from the protonation of the neutral semiquinone radical specie will result in a 1-electron-2-centre bond which are energetically unstable.27 Aside from one study, majority of the studies till date, have proved the existence of semiquinones by observing EPR signals using submitochondrial particles (SMPs). As the name suggests, these are inverted membrane vesicles housing the entire electron transport chain containing all enzyme complexes.34 However, since quinone cofactors are used by majority of the other complexes, distinguishing the semiquinone signals with each complex, has been far from successful. More recently, there has been a wave of research focusing on the identification of semiquinone radicals exclusively from complex I, however these have proved even more challenging as the organic intermediates produced very low intensity signals. Within complex I, there are two species of semiquinone that have been identified; SQNf and SQNs.35,36 Based on their EPR properties, SQNf or fast relaxing semiquinones has been reported only during the presence of an established proton gradient across the membrane. On the other hand, SQNs or slow relaxing semiquinones, are not effected by proton gradient. The presence of two semiquinones has also lead to the possibility of complex I to contain two separate quinone binding sites; Due to SQNf having a spin-spin interaction with Fe-S cluster N2, it is theorised that SQNf binding site is located close to the cluster at around 12 Ã… estimated distance, in contrast, SQNs binding site is suggested to be located around 30 Ã… from N2 cluster.22,25,37 Within the complex, the SQNf is believed to be involved in proton pumping and its site aids the system by acting as bound co-factor site that facilitates the transfer of one electron from one site to another allowing the formation of a binding pocket for the SQNs in equilibrium with the ubiquinone pool of the membrane.22,25,32,35,38 The presence of two separate quinone binding sites still remains a mystery and cannot be totally ruled out even though it has been suggested that SQNf and SQNs signals are detected from the same semiquinone species located from different sites or present in catalysis states.39 A recent potential way of observing semiquinone intermediates via EPR is through the use of liposomes. Liposomes containing just Complex I or proteoliposomes, will facilitate the capture of semiqinone within its native environment and hopefully provide an insight in the mechanism of Complex I and the binding of Q10. Liposomes Liposomes are spherical nanovesicles used in a variety of applications. Composed of a phospholipid bilayer, these small vesicles have an aqueous solution core surrounded by a hydrophobic membrane. Hydrophobic chemicals associate with the bilayer while the hydrophilic solutes dissolved in the core cannot readily pass through the bilayer; essentially mimicking the cellular phospholipid bilayer. Due to these features, liposomes can be loaded both with hydrophobic or hydrophilic molecules and are excellent drug carriers or in this case house protein complexes. Liposomes are also not naturally occurring and must be artificially generated using lipid extracts by aggregating them.40 As liposomes are formed from naturally occurring lipids of low intrinsic toxicity, they are biodegradable and non-toxic. The functionality of liposomes is dependent based on three main factors. These include: size, bilayer composition and liposome surface properties.40 Phospholipids are one the essential components in the formations of liposomes and can be divided into synthetic and natural phospholipids. They consist of two fatty acids hydrophobic chains linked to a hydrophilic (polar) head group, and they have either glycerol or sphingomyeline as the back bone. Having both hydrophobic and hydrophilic components, make phospholipids having amphipathic molecules.41 The diversity of the hydrophilic head group molecules and hydrophobic chains length allows the formation of different phospholipids which affects the surface charge and bilayer permeability of the liposomes.40 The length and degree of saturation of the hydrocarbon acyl chains determines the stability of the liposomal membrane, by affecting the temperature at which the membrane changes from a closely packed gel phase to a fluid phase. The surface charge of the liposomes is determined by the charge of the lipid forming it which can be altered by modifying lipids with hydrophilic moieties to membrane bilayers.40 Liposomes can be composed of naturally-derived phospholipids such as cholesterol, one of the commonly used lipids in liposome formation. It enhances the stability of the lipid bilayer and form highly ordered and rigid membrane with fluid like characteristics. Other phospholipids, synthetic and non-synthetic, can also be used for the formation of the liposomes such as pure surfactant components like DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine).42 Classifications of liposomes Liposomes are classified according to their morphological sizes and lamellarity, depending on their composition and method of formation.40 Multilamellar vesicles (MLVs) consists several concentric phospholipid bilayers or lamellar ranging between 100nm to 20  µm in size depending on the method of preparation. These large bilayers allow the integration of lipophilic molecules and proteins. Small unilamellar vesicles (SUVs) single phospholipid bilayer and sized between 20 nm to 100nm. Ideal for encapsulation small compounds and proteins. Large unilamellar vesicles (LUVs) single phospholipid bilayer with size ranging from 100 nm to 1  µm. They are known to have larger aqueous core compared with or MLVs, making them suitable to useful to load with numerous compounds. Oligolamellar vesicles (OLVs) vesicles similarly structured to MLVs but consists of anywhere between two and five phospholipid bilayers. Multivesicular liposomes (MVLs) When a large liposome vesicle similar in size to an MLV, enclose a group of liposomes, then the subsequent vesicle is known as multivesicular liposome (MVL). Figure 1.40 The current state of research on liposomes have primarily been focusing on the administration of drugs and other compounds to biological systems since it overcome challenges associated with reaching the target, making them very useful in the cosmetic and pharmaceutical industries.40 Furthermore, it should be noted, some surfactant based phospholipids can mimic the biological systems helping construct important model systems for the research on enzymes and membranes. Many recent publications concerning liposomes have been focused on using this mimetic chemistry, which deals with models, mimicking cellular membrane to facilitate the research into their structures as well as the mechanisms both in vivo and in vitro.40 Aims of Project The current state of research on complex I remain largely focused on the determination of the mechanism since only a fraction has been found. Fully understanding will help solve many diseases and other complication caused by complex I. Whereas the mechanism of the reactions between NADH and iron sulphur clusters have been established, little is known about the mechanism of proton translocation as well as the role and existence of semiquinones that will lead into revealing more information into the function of the enzyme. The work described in the following records, using the best technique available, EPR, will aim to be using current studies of using liposomes to mimic cellular conditions, similar to the mitochondrial membrane, for complex I in order to obtain data regarding reduction of Q10 and proton translocation. Materials Preparation of Complex I from Bovine Mitochondria Preparation of Complex I proteoliposomes Stock solutions of 25 mgmL-1 of POPC in chloroform was transferred to a glass homogeniser with the required amount of ubiquinone-10 contained in chloroform. The chloroform was removed under Argon. An alternative approach is to remove under vacuum using rotary evaporator. The resulting phospholipid film was resuspended in 675 ÃŽÂ ¼L of buffer (10 mM Tris-SO4 (pH 7.5) and 50 mM KCl), and extruded 25 times through a Whatman 0.1 ÃŽÂ ¼m pore membrane. The liposome mixture was solubilised with the addition of 160 ÃŽÂ ¼L of octyl-glucoside from an aqueous 10% stock solution, sonicated for 10 min, and further incubated on ice for 10 min. The following steps were carried out at 4  °C. 0.2 mg of AOX (50 ÃŽÂ ¼L of 7.8 mgmL-1) and 0.2 mg of complex I (10 ÃŽÂ ¼L of 20 mgmL-1) were added to the solubilised lipids and incubated for a further 10 min, followed by the addition of 100 ÃŽÂ ¼L of SM2 Biobeads. The mixture wa

Avon Case :: essays research papers

Recommendations for Avon Case Two recommendations for promoting direct-sales through consumer and the business; One is to offer incentives to its customers and second is to motivate the business to promoting its products by advertisements, establishing sales competition for employees, participating in conventions, and by endorsing franchise of its products to retail stores. Promoting Avon’s products and high chance of increasing direct-sales is to offer incentives to its consumers. Incentives provide a positive motivational influence to encourage and excite buyers to buy the products. Such incentives, appropriate for this situation, are coupons, rebates, product samples and awards. Coupons can be attached in mail, beauty magazines, newspapers, and advertisements on mail or on the internet company’s official website. Rebates can be offered also to attract more buyers to buy the products. Rebates can be advertised also on coupons through mail or the internet. Another successful way of attracting consumers is by offering samples of Avon’s cosmetic products, which can be done door-to-door or attached on an advertisement through mail. Finally, awards through prizes, contests or sweepstakes can offer consumers the chance to win something small or big like cash or a free-trip to the Bahamas. Also, prizes can be offering a free gift wheneve r a consumers purchase one of Avon’s products. This last incentive option attracts more attention from consumers than the three other options. Motivate the Avon business to reach its goals of promoting its products and gaining sales through advertisements, sales competition, participating in conventions and endorsing franchise. Advertising is the key to attracting consumers and the outcome is high sales. Such advertisement is done through media (television), mail, or the internet. The more awareness from the public of the company the more likely to attract more buyers and the outcome is high sales.

Criminological Theories Essay -- Legal Issues, Crime

Many of the traditional criminological theories focused more on biological, psychological and sociological explanations of crime rather than on the cost and benefits of crime. More conservative approaches, including routine actives, lifestyle exposure and opportunity theories have clearly incorporated crime rate patterns as a fundamental part of analyzing the economics of crime. Crime statistics are important for the simple reason that they help put theories into a logical perspective. For example, a prospective home owner may want to look at crime rates in areas of potential occupancy. On a more complex level, it helps law enforcement and legislators create effective crime reduction programs. Furthermore, it also helps these agencies determine if crime prevention programs, that have been in effect, have been successful. There are many factors that influence the rates of crime including socio economic status, geographical location, culture and other lifestyle factors. More specifical ly, Messner and Blau (1987) used routine activities theory to test the relationship between the indicators of leisure activities and the rate of serious crimes. They discussed two types of leisure actives, the first being a household pastime, which primarily focused on television watching. The second type was a non-household leisure event which was consisted of attendance to sporting events, cinemas, and entertainment districts. The focus of this paper will be to study the effects that substantial amounts of leisure activities have on the offender and the victim. Leisure activities not only make a crime more opportunistic for offenders, it may also provide offenders with motivation to engage in criminal activity. On the other hand, it may also be argue... ... such as walking in well lit areas, abstaining from areas know as â€Å"hot spots†, and letting someone know your whereabouts, are suggested. Also, these individuals should be aware of the risks that come with certain types of activities. In conjunction to awareness, individuals should be involved in family leisure activities so that their idle hands do not become the devils workshop. Parents provide the primary foundation in the children’s socialization; if this process is not effective or neglected the result could be detrimental. It may lead children to transition into adulthood without the fundamental skills that prevent the development of a criminal disposition. Now that routine activities theory has created a clear understanding of the economics of crime, let’s focus our research on which parenting methods could prevent criminality or crime before if ever develops.

A Comparison of Two Advertisements :: Papers

A Comparison of Two Advertisements Advertisements are messages that are intended to influence and persuade their audience. Their purpose is to raise awareness of the existence of their product in the people whom they target and to promote the benefits of buying or using it. Adverts are paid for by the advertisers and is a major source of income for magazines - approximately 40% of revenues. Without money from advertising, much of the modern media would not exist. Therefore advertising is a commercial business as opposed to ‘free’ publicity like press releases and photo opportunities. A range of advertisers exist in the UK. Many of them are small companies who are trying to promote themselves. Bigger companies will buy in an advertising agency to create the messages in relevant media. Also, the Government spends considerable amounts of money on advertising. They produce two types of advertisements; information (i.e. tax returns) and persuasive (i.e. not to drink drive). I am going to compare and analyse two advertisements from magazines. Magazines have a ready-made pre-defined target audience, so they provide an effective point of contact for advertisers and their target consumers. Both of my chosen adverts are from ‘Bliss’, a teenage magazine aimed at girls roughly aged 11-19. It is the second biggest selling teen title and leads the market in editorial innovation and exciting new ideas. The messages of this type of mainstream magazine are dedicated to the ideal image of a teenage girl who is independent, sexy and looks after her appearance. Fashion, cosmetics and beauty tips take up 50 pages of the magazine, so a substantial part of it is devoted to the improvement of the body and looks. Because image dominates the magazine so much, it seems the ideal medium for advertising lip products. The first advert I have chosen to analyse is advertising a new lipstick. My second advert is promoting is a lip gloss. I am going to scrutinize each advertisement separately, examining how the techniques they have used persuade the consumer to purchase the product, and then

Mghf

Huntington’s disease is an autosomal dominant (Harper et al, 1991) neurodegenerative disorder characterized by involuntary movements, cognitive loss, and psychiatric problems as described by Martin and Gusella (1986). These symptoms are related to the death of medium spiny projection neurons in the caudate nucleus, putamen, and the cortex (Reiner et al, 1988). In later stages of the disease, even areas such as the hippocampus and hypothalamus gets affected as well (Kassubek et al, 2004). Huntington’s disease has a prevalence rate of approximately 1 in 10,000 Caucasian populations (Harper et al, 1991).Patients with Huntington’s disease most typically display a choreic movement disorder involving involuntary writhing movements observed by George Huntington himself. The age of onset of Huntington’s disease seem to be normally distributed around the average age of 35 to 42, with small number of cases developing in patients younger than 20 years of age or older than 60 years of age (Andrew et al, 1993). The majority of juvenile patients, whose disease onset are at age 20 years or less, have inherited the paternally defected gene (Andrew et al, 1993).Patients usually died within 15 to 20 years of disease onset. Gusella et al (1983) first determined the location of the genetic mutation in Huntington’s disease to be the short arm of chromosome 4. It was after another 10 years did the researchers of The Huntington’s Disease Collaborative Research Group (1993) able to discover that a gene in chromosome 4 containing a trinucleotide repeat of CAG was abnormally expanded in diseased individuals. The IT15 (interesting transcript 15) gene, dubbed the huntingtin gene by the group, codes for the huntingtin gene.This CAG repeat, which translates into a poly-glutamine stretch, is highly polymorphic among the population ranging from 11 to 34 copies on normal individuals. However, in individuals with Huntington’s disease it expanded to more than 42 repeats and increasing to upwards of 100 (The Huntington’s Disease Collaborative Research Group, 1993). This provides evidence showing that the mutant huntingtin protein seems to be toxic to its native cells and confers a disease state to individuals with an extended length of repeats. There is also a correlation between the CAG length and disease onset as shown by Andrew et al (1993).The mutated elongated huntingtin protein is cut by enzymes into fragments and the fragments begin to form abnormal clusters, neuronal intranuclear inclusions (NIIs), inside cells. These clusters can also act to recruit normal proteins to adhere together as well (Davies et al, 1997). This was originally thought to cause the pathogenesis of Huntington’s disease. However, more recent studies have shown that the presence of NIIs is actually a coping response to the toxicity of mutant huntingtin proteins and acts to prolong the life of the cells and reduce intracellular mutant huntingtin in neighbouring neurons (Arrasate et al, 2004).The exact function of the wild-type huntingtin protein are unclear, however many efforts have been made in understanding its native functions. Nasir et al (1995) showed that homozygous huntingtin homologs in mice died before embryogenesis could occur and that heterozygotes displayed similar deficits as diseased human patients. Wild-type huntingtin is also crucial for establishing and maintaining neuronal identity, especially in cortex and striatum (Reiner et al, 2001).Current data can provide the conclusion that normal huntingtin protein has actions important for development in mammals. In vitro, wild-type huntingtin have been shown to act to protect brain cells from apoptotic stimuli, such as serum deprivation, mitochondrial toxins, or the transfection of death genes (Cattaneo et al, 2005). Wild-type huntingtin protein, not mutated, stimulates brain-derived neurotrophic factor (BDNF) production by acting at level of Bdnf tra nscription. BDNF is very important for survival of striatal neurons (Cattaneo et al, 2005).Intracellularly, huntingtin protein has been found to associate with various organelles such as the nucleus, endoplasmic reticulum, and Golgi complex (Cattaneo et al, 2005). It has also been found in neurites and at synapses, where it associates with vesicular structures and microtubules (Li et al, 2003). This characteristic has been shown to enhance vesicular transport of BDNF along microtubules (Gauthier et al, 2004). On a similar note, huntingtin interacts with a number of cytoskeletal and synaptic vesicle proteins that are essential for exo- and endocytosis at synaptic terminals.Wild-type huntingtin binds directly to the Src homology 3 domain of postsynaptic density protein 95, which binds NMDA and kainite receptors. This activity is decreased in mutant proteins and can lead to overactivation or sensitization of NMDA receptors (Cattaneo et al, 2005). Aside from the toxicity of the mutated huntingtin protein, the loss of normal huntingtin protein also seems to add to the pathogenesis of Huntington’s disease. Presence of only mutant huntingtin protein results in massive apoptotic cell death in the testes of male mice (Leavitt, 2001).However, no apoptosis can be seen in testes of mice expressing human mutant huntingtin when wild-type huntingtin is expressed as well (Leavitt, 2001). It was also seen that in mice, the absence of wild-type huntingtin protein led to a worsening of striatal atrophy and neuronal loss, and a significant decrease in neuronal cross-sectional area compared to mice that had wild-type huntingtin present (Cattaneo et al, 2005). Huntington’s disease still remains incurable to this day. However, many treatments are available for treatments of its symptoms.Chorea, the hallmark of the disease is a major target for many treatments. Such drugs include dopamine-depleting agents, dopamine antagonists, benzodiazepines, glutamate antagonists, ac etylcholinesterase inhibitors, dopamine agonists, antiseizure medications, cannabinoids, lithium, deep brain stimulation and fetal cell transplantation (Frank and Jankovic, 2010). One notable drug currently in use is Tetrabenazine, which is the only US FDA-approved drug for treatment of Huntington’s disease (Frank and Jankovic, 2010).The drug acts by reversibly inhibiting the central vesicular monoamine transporter type 2, this cause a depletion of dopamine (Bagchi, 1983). The main area of effect for tetrabenazine is in the caudate nucleus, putamen, and the nucleus accumbens, all areas known to be responsible for the major pathology of the disease. The Huntington Study Group (2006) was able to demonstrate the efficacy of tetrabenazine in a double-blind, placebo-controlled trial. Subjects who received tetrabenazine showed a change from the baseline in the maximal chorea score of the UHDRS.Compared to the baseline, treatment resulted in a reduction of 5. 0 units in chorea compa red with a 1. 5 unit reduction for the placebo group. A second symptom targeted for treatment to allow patients to function normally is the psychiatric problems. Dopamine receptor blocking agents are commonly used as anti-psychotics in order to treat psychosis associated with Huntington’s disease (Frank and Jankovic, 2010). There are many ethical issues that have been associated with Huntington’s disease patients, in particular pertaining to the application of genetic testing.The expanded CAG repeats associated with Huntington’s disease (Gusella et al, 1983) are used as a predictive testing to determine the risk of a person for developing inherited HD gene. The debate of ethics with respect to genetic testing for Huntington’s disease seems to lie on several major factors including: autonomy, beneficence, confidentiality, and justice (Huggins et al, 1990). The question remains whether or not the current use of genetic predictive testing is ethical. Autonom y refers to the respect for the individual’s right to make an informed decision about an action that may have a profound effect on his or her life.The patients should feel no pressure from physicians or institutions with regards to their decision for genetic testing. Also, they should also have a full understanding of the consequences and implications of their decisions. This would require physicians to provide all the necessary information to the patients so that they are informed about the testing. If a woman refuses to get tests done for her baby even though her relatives are with her insisting on getting it performed, the physician cannot allow the testing to be performed.Beneficience is summarized by the phrase â€Å"first do no harm† (Huggins et al, 1990). This is an important factor when the results of the test may reveal genetic information about oneself. This implies not only avoiding harm to patient but also preventing harm to other individuals, which may inc lude family members of patients. Due to the genetic characteristics of Huntington’s disease, family members can be both directly and indirection influenced by the results of the test.If for example a pregnant woman decides to get an ultra sound for her baby in order to find out if the baby has spina bifida, and upon confirmation by the physician decides that she wants to give birth at home as opposed to in a hospital where the baby could be treated correctly in order to minimize the risk of the disease. The physician should take any action he can in order to avoid any risk to the health of the baby. Confidentiality with genetic tests applies the same as with any other form of personal information. It should not be disclosed to any other third parties, which includes family members as well.For example, if two siblings were to go and receive a CAG repeat test at the same time and one sibling asks the physician about the results of the other sibling. The physician cannot disclos e any information or they will violate the confidentiality of the patient. Justice simply means equal access to health services and information to all. This also includes long-term support and guidelines for testing as a medical service so that the service is not difficult to obtain if needed. For example, even though Cystic Fibrosis is mostly only prominent in Caucasians, the testing should still be offered to regnant women of other ethnicities as well. Other ethical dilemmas still exist with current genetic testing technologies. One particularly pertaining to Huntington’s disease is whether or not the information is obtained even though there is no cure for the disease. In diseases such as phenylketonuria (PKU), after newborn screening, dietary interventions will allow the individuals with the condition to lead healthy and normal lives (Lea et al, 2005). However, for Huntington’s disease, even after knowing that the disease will affect the individual what can be done ?Therefore, would it be better to not know at all? By testing and finding out that an individual has the mutation that will eventually lead to the development of Huntington’s disease, the individual can attend to the initial development of symptoms and as a result may be treated much earlier. This can result in a more favourable prognosis. Similarly, the individual can live more cautiously as to prevent further exacerbation of their condition. On a more subjective level, the individual may use the knowledge to live a more fulfilling life knowing that he or she may not live as long as the rest of the population.Conversely, the information can also be used negatively. There can be a lot of discrimination in terms of applying for employment and health insurance. This information would also impact the ambition and life goals of the individual, the amount of psychological harm that comes with eventually developing an incurable and lethal neurodegenerative disease is extremely larg e. Similarly, would they still pursue to have a family and children if they knew that they had a very high risk of dying at a young age? This particular issue should be resolved by following the ethical factors listed before.As long as the individual is informed, are being prevented from harm, information not shared with any third party members, and has access to healthcare services, then being tested for Huntington’s disease will be ethically correct. This knowledge will give them insight on how to live the rest of their life. Another dilemma that has Harper, P. S. , Morris, M. J. , Quarrell, O. , Shaw, D. J. , Tyler, A. , and Youngman, S. (1991). The epidemiology of Huntington’s disease. J. Med. Genet. 89: 365-376. Martin, J. B. , and Gusella, J. K. (1986).Huntington’s disease: pathogenesis and management. N. Engl. J. Med. 315: 1267-1276. Andrew, S. E. , Goldberg, Y. P. , Kremer, B. , Telenius, H. , Theilmann, J. , Adamn, S. , Starr, E. , Squitieri, F. , Lin, B. , Kalchman, M. A. , Graham, R. K. , and Hayden, M. R. (1993). The relationship between trinucleotide (CAG) repeat length and clinical features of Huntington’s disease. Nat Genet. 4: 398-403. The Huntington’s Disease Collaborative Research Group (1993). A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington’s disease chromosomes.Cell. 72: 971-983. Gusella, J. F. , Wexler, N. S. , Coneally, P. M. , Naylor, S. L. , Anderson, M. A. , Tanzi, R. E. , Watkins, P. C. , Ottina, K. , Wallace, M. R. , Sakaguchi, Y. A. , et al. (1983). A polymorphic DNA marker genetically linked to Huntington’s disease. Nature. 306: 234-238. Reiner, A. , Albin, R. L. , Anderson, K. D. , D’Amato, C. J. , Penney, J. B. , and Young, A. B. (1988). Differential loss of striatal projection neurons in Huntington disease. Proc. Natl Acad. Sci. USA. 85: 5733–5737. Kassubek, J. , Gaus, W. , and Landwehrmeyer, G. B. (2004).Evidence for more w idespread cerebral pathology in early HD: and MRI-based morphometric analysis. Neurology. 62: 523-524. Nasir, J. , Floresco, S. B. , O’Kusky, J. R. , Diewert, V. M. , Richman, J. M. , Zeisler, J. , Borowski, A. , Marth, J. D. , Philips, A. G. , and Hayden, M. R. (1995). Targeted disruption of the Huntington’s disease gene results in embryonic lethality and behavioral and morphological changes in heterozygotes. Cell. 81: 811-823. Cattaneo, E. , Zuccato, C. , and Tartari, M. (2005). Normal huntingtin function: alternative approach to Huntington’s disease.Nat Rev Neurosci. 6: 919-930. Li, J. Y. , Plomann, M. & Brundin, P. (2003). Huntington’s disease: a synaptopathy? Trends Mol. Med. 9: 414–420. Reiner, A. , Del Mar, N. , Meade, C. A. , Yang, H. , Dragatsis, I. , Zeitlin, S. , and Goldowitz, D. (2001). Neurons lacking huntingtin differentially colonize brain and survive in chimeric mice. J. Neurosci. 21: 7608-7619. Gauthier, L. R. , Charrin, B. C. , B orrell-Pages, M. , Dompierre, J. P. , Rangone, H. , Cordelieres, F. P. , De Mey, J. , MacDonald, M. E. , Lessmann, V. , Humbert, S. , and Saudou, F. 2004). Huntingtin controls neurotrophic support and survival of neurons by enhancing BDNF vesicular transport along microtubules. Cell. 118: 127-138. Leavitt, B. R. , Guttman, J. A. , Hodgson, J. G. , Kimel, G. H. , Singaraja, R. , Vogl, A. W. , and Hayden, M. R. (2001). Wild-type hungtingtin reduces the cellular toxicity of mutant huntingtin in vivo. Am. J. Hum. Genet. 68: 313-324. Davies, S. W. , Turmaine, M. , Cozens, B. A. , DiFiglia, M. , Sharp, A. H. , Ross, C. A. , Scherzinger, E. , Wanker, E. E. , Mangiarini, L. , and Bates, G. P. (1997).Formation of neuronal intranuclear inclusions underlies the neurological dysfunction in mice transgenic for the HD mutation. Cell. 90: 537-548. Arrasate, M. , Mitra, S. , Schweitzer, E. S. , Segal, M. R. , and Finkbeiner, S. (2004). Inclusion body formation reduces levels of mutant huntingtin an d the risk of neuronal death. Nature. 431: 805-810. Frank, S. , and Jankovic, J. (2010). Advances in the pharmacological management of Huntington’s disease. Drugs. 70: 561-571. Bagchi, S. P. (1983). Differential interactions of phencyclidine with tetrabenazine and reserpine affecting intraneuronal dopamine.Biochem Pharmacol. 32: 2851-6 Huntington Study Group. (2006). Tetrabenazine as antichorea therapy in Huntington disease: a randomized controlled trial. Neurology. 66: 366-72 Huggins, M. , Bloch, M. , Kanani, S. , Quarrell, O. W. , Theilman, J. , Hedrick, A. , Dickens, B. , Lynch, A. , and Hayden, M. (1990). Ethical and legal dilemmas arising during predictive testing for adult-onset disease: the experience of Huntington disease. Am. J. Hum. Genet. 47: 4-12. Lea, D. H. , Williams, J. , and Donahue, M. P. (2005). Ethical issues in genetic testing. J. Midwifery Womens Health. 550:234-240.

The Pearl Characters Pen Portrait

Pen Portraits-The beadwork Juana- Juana is a very young maam and she has just had her first child. In the first of the book, she is very calm and does not pronounce much. Towards the end of the book she is a administer more aggressive and speaks more than she utilise to. Her attitude changes and she clings by kino gum whatever happens. An good example is when kino has killed a patch she helps him screen the dead consistence instead of telling anyone of the crime. Steinbeck also writes that when she carries break her way of curing Coyotito by lay the poultice on his sting, she does not leave any faith in her proficiency and thinks that they should go to the convolute.This is the proof that tells us that she has a powerful belief in authority. At the end of the book, she becomes very stubborn and this mulishness leads to her sons death. When kino tells Juana that she should hide with Coyotito to stay safe, she says no three multiplication without any hesitation or w eakness. This is an example of her stubbornness. If she would swallow hidden with Coyotito, the consequences could read differed. My sound judgment of Kino is that she was a very strong character and had a very turgid surcharge to play in this book.Her roll was not only protecting Coyotito, still she was also Kinos largest support. Kino- Kino is a young man who thinks he will achieve everything after he gets the tusk. He has too many ambitions much(prenominal) as sending his son to school, acquire married, and having a rifle. He tells his ambitions to all the villagers and the priest becomes even covetousnessier after hearing what he can do with just a Pearl. Although the priest cannot do anything wrong as he is a religious man, the doctor can. He also sets himself some ambitions such(prenominal) as drinking wine in a rich restaurant.But to achieve his ambitions he must comport the pearl. He tries to mold it further is not successful. Kino knows that on the way to achi eving his ambitions he will have to face evil exactly he is too greedy and the greed offs him blind. tout ensemble he can see atomic number 18 his ambitions. His foolishness is also a savvy of his sons death. If he would have sold it for 1500 pesos than his sons death could have been avoided, but as I have written, greed can make a psyche blind. In my opinion, Kino was trapped by the pearl and he can do nothing to get rescue himself from the pearls camouflaged trap.It is not every day that a man gets lucky. And when Kino gets lucky, he wants to make too much from the luck and this teaches him a lesson you should always be happy with what you have. Juan Tomas- Juan Tomas is Kinos bigger comrade and his wife is Apolonia. Juan Tomas warns Kino about the consequences of what the pearl could do. Kino does not listen to his fellow and very unfortunate consequences follow. For example, Juan Tomas says there is a devil in this pearl but Kino knows this but instead of throwing it aw ay, he keeps it as a hope, he keeps it as his soul.When Kino kills a man in self-defense, Juan Tomas and Apolonia hide Kinos family. This shows us that Juan Tomas nature is very helpful although he knows that he is helping a unlawful who could be sent to jail or even executed, he remembers that he is Kinos brother and should always stay as a support for him and his family From my opinion, He is a very humble man and seems happy with his post in his intent yet, he understands Kino and why he wants to earn too much from the pearl. Juan Tomas, although he has a very short range to play, it is still a very grand one.

The Role of the Media in the Violation of Children’s Rights

Children work the mighty to honest schooling from the media .. which.. should non raise materials that could aggrieve them. (UNICEF, 1991). thither is plain e re on the wholey ram dispel primed(p) on the odious things that the media has to strait and so clawren these geezerhood demand short glide pathion to completely told that they should non be eyeight or consultation in the media. This simply delegacy that the media ar non compete their determination where defend nipperren is concerned.In instantlys lodge where the hoi polloi media comport totally constitute of electronegativity whether on the picture, meshwork, picturegames or even the radio, members of nightclub bring to create certified of what the younger genesis ar macrocosm influenced by so that value that ar taught atomic number 18 non muzzy easily. The media enrapture the rights of a kid by exposing them to wickedness and force disclose, talent them memory gate to sexually app atomic number 18nt means and by creating a mendacious intellect of creation in which they conceptualise or so e genuinelything they bring d stimulate. The media shit barbarianren to plague and furiousness on a day-by-day basis.This is so be stir commonplace at least(prenominal) peasant travel along the video, mind to the radio, nobble video games or breaker the inter sort out. Huesman (1986) as cited in Kundanis (2003) is of the mentation that children with slimy pedantic skills be to a greater extent self-assertive and ar the ones who catch up with practically craze on the telly. It is studyd that somebodynel in the media acquits to hard-hitting doings. (Abel, 2005, Huesmann, 1986 as cited in Kundanis, 2003 & Johnson, 1998). all told of these authors consider that if children atomic number 18 undefended to ferocity in the media it allow nonplus a proscribe feign on them in which they allowing engender aggressive.Chil dren who guide precipitously track down to enthral this behaviour with them to matureness and nearly eons cause molest to those nearly them. deadeningonize to Johnson (1998), children go out(p) what they regain in the tv. nearly of the things that children minute out ar the negativities. These include sess, cleanup spot, conflict and killing dear to clear a few. consort to Johnson (1998) galore(postnominal) a(prenominal) gestate that the p bents ar the ones to be goddamned for children world clear to abuse and violence in the media. only p arnts provide do so much and no much than to mould a menstruum to that.This is because, non all the condemnation pass on they be unsloped or so to superintend their childrens observance habits, the attri to a greater extentovere of medicine they get wind or the websites they visit. It is hold that the nominate work on a reference in restricting it but not to the full phase of the moon extent. It is the medias duty to protect children from materials that could maltreat them (UNICEF, 1991), as yet they ar not play their part and so children atomic number 18 at risk. Children take on to be protect against anything that pass on in least focusings fascinate their qualification to obtain up with the indispensable moral philosophy and values. cosmos open(a) to smut fungus ordain in many way barricade that. found on a research make by Ybarra and Mitchell (2005) 90% of the children surrounded by the ages of 12 and 18 read access to the internet. The more access a child has to the internet, the more subject he or she is to the internet. once the internet conjunctive is there, sexually perspicuous content atomic number 18 scantily a chit-chat away. near(prenominal) generation when children argon glide the net a pop-up secrete window come outs set upment them that they turn out win the draught or sometimes it is in the get of somethi ng very attractive. This is to take them so that they testament be comical about what is accident on their screen. spot some forget terminate it, there argon others who will imperil nurture into it not versed what they are acquiring themselves into. nearly of these things lead to full-grown confine. Children are unexpectedly being open(a) to sexual contents in the media (Ybarra & Mitchell, 2005). some children lumber on to the internet with no invention of regard obscenity in time ends up doing just that. The media continues to feat childrens rights by exposing them to porno on the internet, in films and televised programmes (Media scratch of grapple, 2005). regular(a) though the media set up themselves to ensuring that they do not rape childrens rights they are impuissance in the process. Children conceptualize what they watch on the media, whether it be consecutive or false. This is because the media produces many things that appear to be real. Childr en pure tone to received television characters as their percentage model, whether they champ in a movie, a television exhibition or something of the sort. However, what they split up to draw is that most of the time what their division models are characterisation is not needfully a sizeable thing.To children what is seen on the television is a way of realism (Baran, McIntyre & Meyer, 1984). therefore actions alike(p) smoking are unexceptionable and the touchstone they see on the television is pattern because television is reality. gibe to Berger (2008) seeing is believing, so what a person believe is fastened to what they support seen. whence when a child sees something he or she is reassured that it exists. visual perception alters them to figure with their own eyes that it is in incident the truth. all the same though the Media recruit of Conduct states that they reach themselves to ensuring that the media does not outrage the rights of a child, very brusk is through with(p) to obstruct that. This is so because children are dumb being exposed to all the negativities that the media offers. greater speech pattern involve to be set(p) on protect children from the dangers that are present in the media. This will enable every(prenominal) child to function a lusty life style which they sincerely yours deserve. It is in item the medias fibre to finish their shout out of protect children from the harm and dangers that they offer.